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ROS detection Intracellular production of ROS was measured using DCFH-DA prob

However, these florescent probes are susceptible to various drawbacks such as photo-bleaching and molecular leaching from the host, which may result in serious experimental artifacts 6,7,8,9

Furthermore, enhanced permeability of the blood-brain barrier is a critical neurovascular complication of T2DM, which disrupts CNS homeostasis and impairs its function