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In contrast, direct central mechanisms including PI3K/Akt-mediated neuroprotection, BDNF upregulation, and enhanced autophagy are primarily supported by preclinical data obtained at drug concentrations substantially higher than those achievable in the human brain at therapeutic doses

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Although there was a clear trend for a higher Firmicutes : Bacteroidetes ratio in the WD control group relative to the LD control group, this difference did not rise to the level of statistical significance