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The core plasmid (PLKO.1) and auxiliary plasmids (RRE, REV, Vsvg) inserted into the target gene silencing sequence were used to package the silent lentivirus

Upon binding, the receptor-ligand interaction initiates intracellular signaling cascades, including cyclic adenosine monophosphate (cAMP) pathway activation

We currently have little understanding of the universe of potentially endemic coronaviruses circulating in animal hosts, although a combination of high mutagenic properties and broad host range means that there is a persistent threat of novel zoonotic emergence