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2.13 Mini-G s subcellular translocation assay HEK293-SNAP-GLP-1R cells were seeded in 6-well plates and transfected with 0.2 g plasmid DNA encoding mini-G s -NLuc (a gift from Prof Nevin Lambert, Medical College of Georgia) (Wan et al ., 2018), 0.2 g KRAS-Venus or Rab5-Venus, plus 1.6 g pcDNA3.1, for 24 hours before the assay