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[DOI] [PubMed] [Google Scholar] 2.Lane TF, Iruela-Arispe ML, Johnson RS, et al

Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)

All infections were performed using a standardized inoculum of 1 10 7 colony-forming units (CFU) suspended in phosphate-buffered saline (PBS) and administered via the intranasal route using 50 l/nostril