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Nucleic acid therapeutics: a focus on the development of aptamers

Excess visceral fat in particular drives chronic inflammation

Tris-buffered saline with Tween 20 (TBST) buffer and 3% bovine serum albumin were used to block the polyvinylidene difluoride membrane for 1 h at room temperature to stop the primary and/or secondary antibodies from nonspecifically attaching to the membrane