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1 Introduction Coronary heart disease (CHD) represents a significant threat to global health, characterized by complex and multifactorial pathogenesis ( The pathophysiological significance of ferroptosis in cardiovascular disease stems from its multifaceted involvement in abnormal iron metabolism, lipid peroxidation, and ROS accumulationprocesses fundamental to the development of coronary artery disease (CAD)

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Given that there was no difference in total GSH between Preparation Condition 1 and Preparation Condition 2, and that free GSH is significantly higher in Preparation Condition 1 than in Preparation Condition 2, these results suggest that EDTA-treatment more effectively prevents spontaneous oxidation of free GSH in plasma than does heparinization of samples